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Sample preparation for UHPLC-MS/MS The methanol (1.2 mL) containing the IS solution (10 g/mL, GSH- 13 C 2 , 15 N for negative ion mode (Neg)) was added to the cell-cultured plate and maintained for 2 min

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Subsequently, GO analysis showed that the 94 ferroptosis-related genes have different biological effects, such as the regulation of the glutathione biosynthetic process and cellular response to thyroxine stimulus (biological process)