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doi: 10.3390/nu12102929

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Life 11:353 Pae M, Wu D (2013) Immunomodulating effects of epigallocatechin-3-gallate from green tea: mechanisms and applications

For the neutral conditions, the slides were equilibrated for 20 min in an ice-cold buffer containing 100 mM Tris and 300 mM sodium acetate, with pH adjusted to 9.0 using glacial acetic acid