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24 Besides, NADPH acts as the reducing reagent for 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGCR), the rate-limiting enzyme of the mevalonate pathway, which leads to the synthesis of cholesterol and nonsterol isoprenoids

Alzheimers Dement Transl Res Clin Interv 2:141155 Lewis J, Melrose H, Bumcrot D, Hope A, Zehr C, Lincoln S, Braithwaite A, He Z, Ogholikhan S, Hinkle K, Kent C, Toudjarska I, Charisse K, Braich R, Pandey RK, Heckman M, Maraganore DM, Crook J, Farrer MJ (2008) In vivo silencing of alpha-synuclein using naked siRNA

Transport to the vacuoles ATP-dependent membrane pumps recognize the glutathione S-conjugates and transfer them into the vacuoles Glutathione conjugation tags the xenobiotic substrates for recognition by membrane pumps Measurement of total glutathione S-transferase (GST) activity:spectrophotometrically at 340 nm conjugate CDNB Best model substrate: 1-chloro-2,4-dinitrobenzene (CDNB) Separation of GST isoenzymes by HPLC in wheat extracts Crude extract (NH4)2SO4 precipitation affinity chromatography on GSH-agarose column RP-HPLC UV detection (214 nm) Old history (1990s): GST isoenzymes in maize Two subunits Homo- and heterodimer molecules constitutive or inducible GSTs today Maize: 42 isoenzymes EST (expressed sequence tag) database BLAST searching (Du Pont Company, USA) Arabidopsis thaliana: 47 isoenzymes McGonigle et al., Plant Physiol

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