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The care of the animals was in accordance with the standard operating procedures of the facility for pharmacological animals and the European Convention for the Protection of Vertebrate Animals Used for Experimental and Other Scientific Purposes (ETS 123)

hence diosgenin and charantin have high volume of distribution and ultimately have high t 1/2 values

Common experimental systems include receptor binding assays, neuronal signaling studies, metabolic animal models, and biochemical pathway analysis