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Peptide lysates were injected on a trapping column (Acclaim PepMap 100 C18, 3 m, nanoViper, 75 m x 2 cm, Thermo Fisher Scientific) with 5 L/min by using 98% water/2% ACN 0.5% trifluoroacetic acid, and separated on an analytical column (Acclaim PepMap 100 C18, 3 m, nanoViper, 75 m x 25 cm, Thermo Fisher Scientific) with a flow rate of 300 nL/min

The endogenous hallucinogen and trace amine N,N-dimethyltryptamine (DMT) displays potent protective effects against hypoxia via sigma-1 receptor activation in human primary iPSC-derived cortical neurons and microglia-like immune cells

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Key Takeaway: The "how many days" question depends largely on delivery