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Single Research Group Dominance: The vast majority of published studies originate from a single research group at the University of Zagreb, Croatia, and the lack of independent replication from other laboratories is a noted limitation across the scientific community

pointed out that pyrimidine biosynthesis could accelerate glycolysis via activating Notch signaling and enhancing the expression of c-Myc, leading to hindering the efficacy of chemotherapy (68)

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Endotoxin Control: Critical for mitochondrial assays where bacterial byproducts could skew inflammatory markers or oxygen consumption rates (OCR)