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[DOI] [PubMed] [Google Scholar] 4.Kolte D., McClung J.A., Aronow W.S

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The activity of alkaline phosphatase was calculated with the following formula: Where: OD T0 = D value at 405 nm at time 0, OD T0 = OD value at 405 nm at time 4 min, RxnVol = reaction volume, PNP = absorptivity coefficient for p-Nitrophenol (18.75 mM 1 cm 1 ), L = light path in cm, SmplVol = sample volume, t = reaction time, 4 min