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10.1016/j.etap.2013.11.021 [PubMed: 24355796] [CrossRef] Horwitz DL, McLane M, Kobe P (1988)

Data availability The datasets are available from the corresponding authors upon reasonable request

After washing the resin repeatedly with kinase buffer (wash fractions), the protein of interest was eluted using 20 mM glutathione (Bio Basics Canada) and concentrated by centrifugation using 15 kDa Centrifugal Filter Units (Millipore)