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Crypts were then mechanically separated from the connective tissue by shaking, and filtered through a 70 m mesh into a 50 mL conical tube to remove villus material and tissue fragments

Assays for TSP-related parameters Met and cysteine were detected using high-performance liquid chromatography (HPLC, Agilent 1200SL, CA, USA) based on a previously reported method with minor modifications 84

This article reviews peer-reviewed evidence on GLP-1 agonists, focusing on mechanisms, applications under study, and limitations, while emphasizing that findings are preliminary and evidence varies in strength

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