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The CCK8 assay was used to detect the proliferative capacity of the cells, MIA PaCa-2 and PANC-1 were spread in 96-well plates at 1500 cells/well, and then transfected after incubation for 24 hours (at this time it was 0 h), About 10 L of CCK8 (TargetMol, USA) solution was added to the 96-well at 24, 48 and 72 h after transfection and cells were incubated in 37 C for 2 h

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g , Mass spectrum of representative INTS12 peptide containing the indicated phosphorylated residue, with both b-ions and y-ions shown in green and yellow, respectively