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After centrifugation at 12700 rpm for 2 min at room temperature, the supernatant was transferred to a new RNase-free microtube containing 0.1 ml of 5 M NaCl, and 0.3 ml of 24:1 (v/v) chloroform-isoamyl alcohol was added, thoroughly mixed, and centrifuged at 12700 rpm for 10 min at 4 C

SREBP2 expression decreased gradually with tumor progression (Fig

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