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All blots were blocked for 30 minutes in 5% milk (dissolved in 1 TBST, TBST: 20 mM Tris pH 7.5, 150 mM NaCl, 0.1% Tween-20) at room temperature and incubated in primary antibody overnight at 4 o C

In a similar manner, the investigation of circadian variation in relation to cholesterol revealed a peak in the evening in three out of four studies [3]

Its toxicity is mostly restricted to the oxidation of highly reactive thiol groups, some of which are functionally very important

CEREBAL (CSICAECID), project INTER23001 and the Spanish government through the Severo Ochoa Center of Excellence Grant CEX2024-001494-S funded by AEI FAFA was supported by a Ramn y Cajal fellowship (Ref