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Purified proteins were dialyzed against PBS, centrifuged at 18,000 g for 20 min to remove aggregates, and protein concentration was determined using NanoDrop spectrophotometry and BCA assay (Thermo fisher, 23225)

Disclosures: Kenneth Sherman: AbbVie: Grant/Research Support, Gilead: Grant/Research Support, Helio: Grant/Research Support, Intercept: Grant/Research Support, Zydus: Grant/Research Support, NovoNordisk: Advisor, MedPace: Consultant, Pliant: Consultant, Horizon/Amgen: Consultant, Minhee Kang: Nothing to Disclose, Triin Umbleja: Nothing to Disclose, Andrea Cox: Nothing to Disclose, Karen Vigil: Gilead Sciences: Speaking and Teaching, Theratechnologies: Grant/Research Support, ViiV: Advisor, Ngan Ta: Nothing to Disclose, Ayotunde Omoz-Oarhe: Nothing to Disclose, Jennifer Price: Nothing to Disclose, Josphat Kosgei: Nothing to Disclose, Leolin Katsidzira: Nothing to Disclose, Hugo Perazzo: Nothing to Disclose, Kevin Knowles: Nothing to Disclose, Beverly Alston-Smith: Nothing to Disclose, Kristen Marks: Gilead Sciences, Inc: Consultant, Icon: Consultant 371 ROLE OF AIR POLLUTION IN THE DEVELOPMENT OF HEPATOCELLULAR CARCINOMA AMONG CHRONIC HEPATITIS B PATIENTS TREATED WITH NUCLEOTIDE/NUCLEOSIDE ANALOGUES Chia-Yen Dai 1 Tyng-yuan Jang 2 Chih-Wen Wang 1 Chung-Feng Huang 2 Ming-Lung Yu 1 , 1 Kaohsiung Medical University, 2 Kaohsiung Medical University Hospital, Kaohsiung Medical University Background: Hepatitis B virus (HBV) infection is the major cause of hepatocellular carcinoma (HCC)

Cryo-EM structures reveal two distinct conformational states in a picornavirus cell entry intermediate

Metabolomic and transcriptomic analysis of the rice response to the bacterial blight pathogen Xanthomonas oryzae pv