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The labeled protein concentrations were adjusted to 100 nM based on fluorescence intensity measured during pretest scans, ensuring signal quality and uniformity across all three Syn constructs for direct comparison of binding affinities

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Post-translational modifications observed in Prx2, such as phosphorylation, proteolysis, and even the hyperoxidation of its peroxidatic cysteine, are involved in the regulation of its peroxidase activity and could affect its participation in redox signaling pathways [87,88,89]

The peptide drug class encompasses everything from blockbuster GLP-1 receptor agonists prescribed to millions of patients, to emerging compounds still building their evidence base in clinical research