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Finally, the samples were mounted with an anti-fade mounting medium containing DAPI (4083, CST) or Hoechst (4082, CST), and images were acquired and observed under a laser scanning confocal microscope (LSM 980, ZEISS)

In addition the authors contend that based on their results mitochondrial O 2 /H 2 O 2 production can be mistakenly associated with Complex I rather than Odh and Pdh (Quinlan et al., 2014)