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To help ensure high purity of the bound target protein, the beads should be washed 2 to 4 times with buffer on the magnet to remove all unbound proteins

Biochem Pharmacol (2010) 80(9):134355.10.1016/j.bcp.2010.07.019 54 ByrneLVan HarenF

10.1016/s0891-5849(01)00480-4 183 SchieberM.ChandelN

However, subsequent investigations revealed that nearly 10% of individuals treated with ESAs develop resistance to erythropoietin, potentially associated with iron insufficiency and inflammatory conditions (Macdougall, 2024)