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2.8 In vitro innate immune profile evaluation B16-F10 cells (5x10 5 cells/well) and BMDMs (5x10 5 cells/well) were infected with BCG Moreau (MOI 5, 10, 20, and 40) or stimulated with specific agonists such as Pam3CSK4 (1 g/ml Invivogen, tlrl-pms), polyI:C (3 g/ml InvivoGen, tlrl-pic), ultrapure Escherichia coli LPS (100 ng/ml InvivoGen, tlrl-3pelps), CpG-ODN (1 g/ml InvivoGen, tlrl-1826-1), dsDNA90 (3 g/ml) or Escherichia coli LPS (1g/ml - Sigma-Aldrich, L6529) plus Nigericin (20M - Sigma-Aldrich, N7143)

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doi: Zhang YH, Yan XZ, Xu SF, Pang ZQ, Li LB, Yang Y, Fan YG, Wang Z, Yu X, Guo C, Ao Q (2020) -Lipoic Acid Maintains Brain Glucose Metabolism via BDNF/TrkB/HIF-1 Signaling Pathway in P301S Mice