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After washing, sections were incubated with fluorescent secondary antibodies for 1 h at room temperature, washed again, mounted, and imaged using Olympus laser scanning confocal microscope (Spin SR) or slide scanner of Zeiss (Axioscan 7)

Therapeutic targeting of metabolic reprogramming in NSCLC Glycolysis inhibitors Targeting glycolysis in NSCLC has been explored as a therapeutic strategy, with compounds such as 2-deoxyglucose (2-DG) and PFKFB3 inhibitors showing promise in preclinical studies as presented in Fig