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Elife 7 , e42166 (2018)

doi: 10.3389/fimmu.2020.01834 281 SimulaLFumagalliMVimeuxLRajnprehtIIcardPBirsenGet al

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Quantitative real-time polymerase chain reaction (PCR) analysis Total RNA was extracted using a TRIzol kit (Invitrogen, Carlsbad, CA, USA) and cDNA synthesis was performed using a cDNA synthesis kit (Fermentas Life Sciences) according to the manufacturer instructions