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Present therapeutic approaches are predominantly symptomatic in nature and fail to halt the process of neurodegeneration, thereby underscoring an urgent imperative for disease-modifying strategies that target protein aggregation, oxidative stress, and neuroinflammatory processes

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Protein purification under denaturing conditions: Proteins that are expressed as an inclusion body and have been solubilized with chaotrophic agents such as guanidine-HCl or urea can be purified by modifying the protocol to include the appropriate amount of denaturant (up to 6M guanidine-HCl or up to 8M urea) in the binding, wash and elution buffers

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