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The reaction mixture was further incubated at 25C for 2 hours, and the solution was transferred to a centrifugation tube where prechilled acetone at 20C was added and the solution was then dissolved in PBS + 1% SDS

doi: 10.1001/jama.2018.0483 119 StockleyR.De SoyzaA.GunawardenaK.PerrettJ.Forsman-SembK.EntwistleN.et al

Type IV pili binding to CD147/Basigin triggers tyrosine phosphorylation through c-Src and focal adhesion kinase, and EGFR is activated (Slanina et al., 2010, Slanina et al., 2012, Slanina et al., 2014), and acid sphingomyelinase increases ceramide levels on the plasma membrane, forming platforms for the recruitment of host receptors (Simonis et al., 2014

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