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Subsequently, the obtained solution was extruded through a 0.05 m pore size membrane (FPB005A12, Membrane Solutions, Shanghai, China) and mixed with si-PGC-1 prepared in 30% ethanol and 50 mM citric acid (pH 4) to achieve a total lipid:siRNA ratio of approximately 7:1 (by weight)

In the clinical trials leading to the approval of these agents (palbociclib and ribociclib) letrozole was chosen to represent the aromatase inhibitor class