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10.1016/j.bbalip.2013.10.018 59 KhnL

As summarized in Figure 1 , the collective evidence demonstrates that heat stress acts across multiple biological levels, affecting whole-animal physiology, microbial ecology, immune responsiveness, and cellular function, resulting in compromised health and immune resilience in dairy cattle

The duration of effects and factors influencing permanence

Gene expression analysis For gene expression microarray analyses, GeneChip Human 1.1 ST arrays were hybridised according to Affymetrix recommendations, using the Ambion WT Expression Kit protocol (Life Technologies) and Affymetrix labelling and hybridisation kits, as previously described in ref