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In case of Drosophila , neuronal redox balance was altered by driving CatalaseRNAi or PHGpxRNAi, along with roGFP by elav-Gal4 to deplete the respective antioxidant enzymes and express the redox biosensor

It not only protects against free radicals, but also has the ability to regenerate other antioxidants consumed by oxidation

Further doseresponse and steady-state administration studies will be required to optimize the dosing schedule for future trials to evaluate therapeutic efficacy

Wash with PBS one more time and add 500 l of lysis buffer (0.1% Triton-X and 0.6% sulfosalicylic acid in 0.1 M potassium phosphate buffer with 5 mM EDTA at pH 7.5) on ice