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The next day, cells were washed twice in pre-warmed Na + -free uptake buffer (137 mM choline chloride, 3 mM KCl, 1 mM CaCl 2 , 1 mM MgCl 2 , 5 mM D-glucose, 0.7 mM K 2 HPO 4 , 10 mM HEPES, pH 7.4), then incubated for 10 minutes at 37 C

Storage: Once opened, store in refrigerator and consume within 45 days

This method of cross-validation trains and tests a model four times using independent sample sets, based on a training set of 75% and a test set of 25% of the samples, with model performance assessed as the average test set performance across repeats

Additionally, this may also support an uncoupling induced increase in NADH reduction and oxidation