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Circadian light entrainment in Drosophila is mediated by the light-sensitive photoreceptor cryptochrome (cry) ( Drosophila , cry responds to blue light by binding to tim and recruiting the E3 ubiquitin ligase jetlag (jet), marking tim for proteasomal degradation and thereby resetting the molecular clock (Stanewsky et al., 1998

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b ) Calibration curve of TRaQ-G with adduct formed by in vitro reaction of purified fusion protein with ligand and measured by fluorescence microscopy