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Cells were lysed with 500 L of RIPA lysis buffer containing 150 mM NaCl, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, 25 mM Tris-HCl, supplemented with protease and phosphatase inhibitors at a 1:100 dilution

10.1016/j.cmet.2020.10.011 210 ZhuZ.WangY.DengZ.LeiP.LiuQ.GuoJ.et al (2024)

The Resin would then turn white without Nickel

This balanced dual formulation allows researchers to investigate synergistic signalling between GHRH and ghrelin receptor pathways in controlled laboratory models