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To investigate how metformin exerted its antioxidant effects, we first examined the expression of Nrf2 and its downstream regulatory protein HO-1 in the chondrocytes exposed to H 2 O 2 , with or without metformin treatment

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SEC buffer for cryo-EM analysis consisted of 0.01% lauryl maltose neopentyl glycol, 18-36 M GDN (anatrace GDN101), 0.001% cholesteryl hemisuccinate Tris salt, 50LmM HEPES (pH-adjusted with KOH, pHL7.5) and 150LmM KCl