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Description

N Engl J Med 2008;359:991

The inoculated leaves were boiled in trypan blue staining solution (10 ml lactic acid, 10 ml glycerol, 10 g phenol, and 10 mg trypan blue dissolved in 10 ml ddH 2 O) for 3 min and stained overnight in chloral hydrate solution (2.5 g chloral hydrate dissolved in 1 ml ddH 2 O)