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Since the interaction of polyhistidine-tagged fusion proteins and MagneHis Ni-Particles does not depend on tertiary structure, fusion proteins can be captured and purified using denaturing conditions by adding a strong denaturant such as 28M guanidine hydrochloride or urea to the cells

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10.1038/s41598-020-62184-8 Summary Keywords vaginal microbiota, inflammation, cytokines, neutrophils, sexually transmitted infections, non-human primates Citation Adapen C, Rot L and Menu E (2022) Role of the human vaginal microbiota in the regulation of inflammation and sexually transmitted infection acquisition: Contribution of the non-human primate model to a better understanding