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Hereafter, 0.5 ml of non-clotted blood was transferred to a 2 ml vial and immediately centrifuged (3,000 g, 10 min), and the supernatant was subsequently removed

10.1016/j.jnutbio.2004.03.004 J Nutr Biochem

Of the total plasma Hcy, 8090% exists in a protein-bound form, approximately 1020% is in an oxidized form, and only less than 1% exists as a free, reduced amino acid [6,7]