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J Physiol Pharmacol.1998:49(2):241-9

As to the K-562 cells, cells were collected directly, washed twice with 500 L 1 PBS, and resuspended in 500 L binding buffer

Hirao, A., Tahara, Y., Kimura, I

Beneficial endocrine but adverse exocrine effects of sitagliptin in the human islet amyloid polypeptide transgenic rat model of type 2 diabetes: interactions with metformin