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As ES cells are pluripotent and nonmalignant, they provide an ideal system to elucidate core mechanisms underlying cellular adaptation to AA stress, independent of oncogenic transformation

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ROS/RNS modulate the activity of a variety of transcription factors, including Apurinic/apyrimidinic endonuclease 1/redox effector factor 1 (APE1/Ref1), Hypoxia-Inducible Factor (HIF1), Activator protein 1 (AP1), nuclear factor erythroid 2related factor 2 (Nrf2), Nuclear factor-kappa B (NFB), p53 gene, forkhead box transcription factors (FOXO), and catenin (57)

The only way to ensure sterility is to adhere strictly to the 28-day rule and proper refrigeration