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Isolation and RTqPCR of total HIV DNA were performed as described earlier (Kessing et al, 2017)

The loss of kinesin-driven axonal transport would likely have many consequences for neurons such as (1) energetic deficits given that cells would have impairments in transport of metabolic enzymes

For SPE, 100 L of 3- tert -butylanisole (2 mM in absolute ethanol, 99.9 %, VWR, Darmstadt, Germany), 10 L of the internal standard 4-methoxy-2-methyl-2-butanethiol (11.2 M in ethanol), and 20 L of ETP (99 %, Merck, Darmstadt, Germany) were added to 50 mL of the sample