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For the observation of mitochondria generating ROS, the cells were incubated with HBSS (Sigma-Aldrich) containing 5 M MitoSOX for 10 min at 37 C in the dark and were then washed with PBS 3 times to remove any residual dye

Materials and Methods Animals Five-week-old female C57BL/6 mice (2025 g) were utilized in this study

Blood 2010;116:1574-84