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and L.P.) and the Wellcome Trust (E.S., E.B

The resulting mixture was filtered, and the filtrate was diluted with water into a calibrated 100-mL flask

This can be done by beginning with examining substances for which there are reports of their potential toxicity to the intestinal barrier and determining the degree of toxicity, monitoring the concentrations in the environment and various products of compounds toxic to the intestinal barrier, as well as limiting contact with substances that may cause intestinal leakage to doses that could be considered safe