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Methods Cell culture HEK293 cells (FreeStyle 293, Thermo Fisher Scientific, #R79007), adapted to serum-free suspension culture, were used for EV production

Protein sequences are the primary and central source of information of the SwissPalm database

Basal OCR was measured, followed by sequential treatment of oligomycin A (1 M), carbonylcyanide- p -trifluoromethoxyphenylhydrazone (FCCP) (0.9 M), and rotenone/antimycin (1 M) (optimal reagent concentrations were determined in preliminary experiments)

ACTIVEMANNO WHITE is an adjuvant of fermentation containing all the nutrients necessary to the development of the natural mycotic present in grape must (essential and complementary amino acids, sterols, unsaturated fatty acids, branched polysaccharides readily assimilated), which are the base of the most qualified preparations made with yeast walls