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The only exceptions were substitution of W7 with tryptophan analogues (within 2x of the parent affinity) and substitution of Cba with Leu (K D = 56 nM) or Ala (K D = 115 nM)

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We assumed that some shRNAs might favor survival of ATP7B-KO cells by suppressing generic cell death genes rather than genes that specifically regulate Cu toxicity