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Label every vial: peptide name, concentration (mg/mL or mcg/mL), reconstitution date, lot number, and operator initials

Proteins were eluted by incubation with 25 L of 100 g/ml 3xFLAG peptide (Sigma) in 0.05% RapiGest (Waters Corp) in IP buffer on an end-over-end tube rotator at 10 rpm at 4 C overnight

Nitric oxide (NO) is recognized as a mediator in a broad array of biologic systems

Since the 1990s, folate status and genetics have been linked to a plethora of diseases, many of which stem from impaired DNA-thymidylate and methylation pathways, and include adverse prenatal, oncogenic, cardiovascular and neurological outcomes