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Biophysically different redox-sensitive fluorescent readouts like the fluorescent lifetime of roGFPiE [23] or the excitation ratio of Grx1-roGFP1-iE ER [25] or an OxyR-YFP fusion protein called HyPer-ER [24] observed the same phenomenon in living cell experiments

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MAST4 shRNA plasmids were made by inserting the annealed shRNA targeting sequences (MAST4-sh1 and MAST4-sh2) behind a U6 promoter in the pCAG-IRES-GFP plasmid