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228 CCND1 is the G1/S checkpoint of the cell cycle, and reduced expression of CCND1 is associated with G1 phase arrest

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The rate can be amplified by transition metals, and we and others have documented metal-driven oxidation of enzyme thiols in vitro and in the bacterial periplasm (Hiniker et al., 2005

coli BL21 were co-transformed with the pEVOL-pBpF plasmid and the expression plasmid carrying the respective Munc18-1 amber mutant