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[DOI] [PubMed] [Google Scholar] 36

This age-related NAD+ depletion impairs mitochondrial energy production, reduces SIRT1 activity, compromises DNA repair mechanisms, and accelerates cellular senescence

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Readouts most often include glycerol release and free fatty acid quantification in the supernatant, alongside cellular ATP or viability measures to confirm that observed lipolysis reflects genuine metabolic signaling rather than cytotoxic membrane disruption