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In addition to causing flu-like symptoms, such as fever, exhaustion and malaise, TNF- also causes lung damage, vascular leakage, heart failure and the production of acute-phase proteins Shimabukuro-Vornhagen et al

This hypothesis was further supported when similar findings were obtained with ferrous iron treatment

In addition, various Mtb knockout strains are employed for infection of guinea pigs, not only to study their role in virulence and survival of the pathogen, but also to validate these virulence factors as important drug targets