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A total of 200 M hydrogen peroxide (H 2 O 2 ) significantly induced cytotoxicity in HeLa cells, while H 2 O 2 up to 300 M did not induce cytotoxicity in HepG2 cells, further demonstrating the strong resistance of HepG2 cells to ROS

Lysates were clarified by centrifugation at 15,000 g for 30 min at 4 C and flowed over pre-equilibrated Pierce high-capacity streptavidin agarose (3 ml of resin)