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Matute DR, McEwen JG, Puccia R, Montes BA, San-Blas G, et al

10.1083/jcb.201503023 56 KlionskyD

developed a software-based technique, which allowed performing morphometric analysis of eryptotic cells evaluating cell size (cell shrinkage), cell irregularities (membrane blebbing), granularity, and central halo (loss of a biconcave shape) [85]

Evaluation of autophagy flux For the autophagy flux analysis using mCherry-GFP-LC3 fusion protein, 30 cells were randomly selected, and the percentage of yellow-positive puncta was equal to that of the ratio of yellow puncta to all LC3 puncta (yellow puncta and red-only puncta)