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Gluten and casein-derived molecules, if present in subjects with altered immune system, like in ASD, can cross the highly permeable intestinal barrier, and activate pro-inflammatory processes, releasing pro-inflammatory mediators that travel to the brain centers through the blood, contributing to neuro-inflammatory activities (Fiorentino et al., 2016)

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In vitro reactive oxygen species (ROS) assay ARPE-19 cells were cultured as above then seeded at a concentration of 2.5 10 4 cells/well (200 L) in fresh culture media in 96-well black walled, clear bottom tissue culture treated plates for fluorescence detection