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Comprehensive Reviews in Food Science and Food Safety, 12 (1), 523

Before derivatization, 2 mL 0.2 M phosphate buffer was added

In addition, Glu has the following features: chemical stability, metabolic generation and easy removal (interconversion with -ketoglutarate), negative charge (at physiological pH value), and acidic amino acid [due to its two carboxyl groups (- and -carboxyl) and one amino group]