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342 When Hedgehog ligands are absent, a Glioma-associated oncogene (Gli) is phosphorylated 343,344,345,346,347 and then undergoes proteolytic cleavage, generating Gli repressor (GliR)

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The dysregulated secretion of adipokines leads to insulin resistance and increased inflammation (406), while the intricate interplay between AT and the immune system becomes crucial in age-related metabolic decline, mirroring patterns observed in obesity (425)

Optional: If the lysate is too viscous, add RNase A (10 g/mL) and DNase I (5 g/mL) and incubate on ice for 10-15 minutes