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To explore the possibility of a direct protein-protein interaction between Ssu72 and GSK3, GST pulldown assays were performed by incubating cellular extracts from differentiated 3T3-L1 cells with purified glutathione S-transferase (GST) or GST-fused Ssu72 (GST-Ssu72) proteins (Supplementary Fig

6a), with a concentration of approximately 1.5 times higher than cells treated with Ru(II)-OH and NP2 (Fig

In this review, we summarize the mechanisms of five major transcription factor familiesWRKY, ERF, MYB, bHLH, and bZIPin plant resistance to Cd stress to provide useful information for using molecular techniques to solve Cd pollution problems in the future