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The first being the pro-inflammatory or classically activated macrophage that responds to bacteria, viruses, lipopolysaccharide (LPS), and interferon gamma (IFN)

The pharmacological form of B12 may also be relevant

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Subsequently, two 500 mL LB broth tubes containing the above mentioned antibiotics were inoculated with 1% (v/v) of 45 h grown culture and incubated at 37 C with shaking